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选择6例健康人(其中3例吸烟、3例无吸烟嗜好)为对象,研究棉酚诱发淋巴细胞SCE频率的影响。实验分为加或不加S-9的0.5,1,3,5,7μg/ml 5个棉酚剂量组,比较吸烟者与不吸烟者的各棉酚剂量组,诱发体外人体外周血淋巴细胞SCE频率的差异。结果表明除例2的1μg/ml,例3的1,7μg/ml等个别棉酚组SCE频率与各自的对照组比较,显示有统计学意义的改变(P<0.05)外,3例大部棉酚组的SCE频率变化,均无统计学意义(P>0.05)。用相同方法,改用二甲基亚砜作溶剂,对上述3例重复实验的结果,亦无显著差异(P>0.05)。有吸烟嗜好的3例正常人中,加S-9的各棉酚组SCE频率,差异非常显著(P<0.001)。不加S-9的各棉酚组,除少数棉酚组SCE频率有明显升高(P<0.001)外,大多数棉酚组SCE频率虽有变化,但无统计学意义的改变(P>0.05)。分析上述结果,棉酚对正常人无诱变作用。吸烟者3例,其SCE频率升高可能与人吸入烟凝结物中的苯并芘等致癌物与S-9相互作用形成环氧化物有关。
Six healthy subjects (three were smoking and three were non-smoking) were selected to study the effect of gossypol on SCE frequency in lymphocytes. The experiment was divided into five gossypol doses of 0.5, 1, 3, 5 and 7 μg / ml with or without S-9. The gossypol dose groups of smokers and non-smokers were compared to induce in vitro human peripheral blood lymphocytes SCE frequency difference. The results showed that in addition to cases 2 1μg / ml, Example 3, 1,7μg / ml and other individual gossypol SCE frequency compared with the respective control group, showed a statistically significant change (P <0.05), 3 cases most Gossypol group SCE frequency changes were not statistically significant (P> 0.05). Using the same method, the use of DMSO as a solvent, the results of the above three cases of repeated experiments, there was no significant difference (P> 0.05). There was a significant difference (P <0.001) in SCE frequency between the three gossypol groups with S-9 and those with smoking preference. In all gossypol groups without S-9, SCE frequency was significantly increased in most gossypol groups except for a few gossypol groups (P <0.001), but there was no statistical difference (P> 0.05). Analysis of the above results, gossypol no mutagenic effect on normal people. 3 cases of smokers, the increase of their SCE frequency may be related to human inhalation of cigarette smoke condensation of carcinogens such as benzopyrene and S-9 formation of epoxide.