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目的:探讨端粒酶活性在肺癌患者诱导痰中表达的临床意义,寻找肺癌诊断的新辅助方法。方法:采用TRAP-PCR-ELISA法对30例肺癌患者诱导痰、自然痰及15例肺部良性病变者诱导痰中端粒酶活性进行测定。结果:肺癌患者的诱导痰、自然痰以及非肺癌患者诱导痰中的端粒酶阳性率和OD值分别为80.0%、56.7%、13.3%和0.523±0.267、0.349±0.247、0.091±0.110。肺癌诱导痰组高于自然痰组及良性对照组,差异有显著性统计意义(P<0.01)。结论:诱导痰中端粒酶活性的检测有助于肺癌的诊断及其与肺部良性疾病的鉴别诊断,且优于自然痰。
Objective: To investigate the clinical significance of telomerase activity in the induced sputum of patients with lung cancer and to search for new adjuvant methods for the diagnosis of lung cancer. Methods: TRP-PCR-ELISA was used to detect the telomerase activity in sputum from 30 cases of lung cancer induced sputum, natural sputum and 15 cases of lung benign lesions. Results: The positive rate and OD value of telomerase in induced sputum, natural sputum and non-lung cancer patients were 80.0%, 56.7%, 13.3% and 0.523 ± 0.267,0.349 ± 0.247,0.091 ± 0.110 respectively. The sputum of lung cancer was higher than that of natural sputum and benign control group, the difference was statistically significant (P <0.01). Conclusion: The detection of telomerase activity in induced sputum is helpful to the diagnosis of lung cancer and its differential diagnosis with benign pulmonary diseases, and it is superior to natural sputum.