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目的: 探讨研究血管紧张素原(AGT)基因 3′端 GT重复序列多态性在不同种族群体中的分布特点。方法: 应用PCR扩增、毛细管电泳和Genetic Profiler 自动分析软件进行微卫星基因组扫描分析技术,检测 170 名新疆哈萨克民族AGT基因的3′端GT重复序列多态性。结果: 新疆哈萨克民族 AGT基因 3′端 GT重复序列存在13种等位基因,按片段由小至大分别命名为A1~A13(GT的重复次数依次为 13~25), 扩增长度为 160~184bp, 其频率为0.003~0.197。以扩增片段长度为 170 bp的 A6 等位基因最为常见,其次为 A3 和 A4,分布符合Hardy Weinberg遗传平衡定律。该位点杂合度为 0.862,多态信息量为 0.847。结论: 新疆哈萨克族人 AGT基因3′端GT重复序列等位基因型与其他人种、民族人群不同。
AIM: To investigate the distribution of GT repeats polymorphism at the 3 ’end of angiotensinogen (AGT) gene in different racial groups. Methods: The microsatellite genomic scanning technique was used to detect AGT gene polymorphism at the 3 ’end of Xinjiang Kazakh nationality by PCR amplification, capillary electrophoresis and Genetic Profiler automatic analysis software. Results: Thirteen alleles were found in GT repeats of AGT gene in Kazak nationality in Xinjiang. The fragments were named as A1 ~ A13 (the number of repeats was 13-25) 184bp, its frequency is 0.003 ~ 0.197. The A6 allele with the length of 170 bp was the most common, followed by A3 and A4. The distribution was consistent with Hardy Weinberg’s law of genetic balance. The heterozygosity at this locus was 0.862, and the amount of polymorphic information was 0.847. Conclusion: The 3 ’GT repeat allele of AGT gene in Xinjiang Kazak people is different from other races and ethnic groups.