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目的探讨炎性信号通路磷酸化信号转导和转录激活因子3(p-STAT)的原发活化状态及下游细胞因子白细胞介素(IL)-17表达在乳腺癌发生和发展中的作用及其对预后的影响。方法运用免疫组织化学Envision两步法检测了379例乳腺癌患者以及匹配癌旁245例乳腺腺病中IL-17表达和原发性p-STAT3的活化状态,分析了它们与乳腺癌组织学分型、TNM分期、临床分期及预后的相关性。统计学分析:计量数据使用t检验法,计数数据使用χ2检验法。结果 1.IL-17在乳腺癌中的阳性表达率为95.8%,显著高于乳腺腺病中的阳性表达率85.3%(χ2=21.363,P<0.001);具有活性的p-STAT3在乳腺癌中的阳性率为93.6%,也显著高于乳腺腺病中的阳性率62.0%(χ2=97.702,P<0.001)。2.IL-17和p-STAT3在乳腺癌(包括乳腺浸润性导管癌、乳腺浸润性小叶癌和腺导管原位癌分型)中的阳性率与乳腺癌组织学分型无相关性(χ2=1.245,P=0.535>0.05)。3.IL-17和pSTAT3在乳腺癌中的强阳性率分别与淋巴结的转移成正相关(IL-17:χ2=7.806,P<0.01;p-STAT3:χ2=4.053,P<0.05)。4.在乳腺癌组织中,IL-17表达与p-STAT3活性呈正相关(rs=0.136,P<0.01)。结论原发增高的p-STAT3活性及高表达的炎性细胞因子IL-17可能协同作用,产生炎性微环境,从而参与乳腺癌的发生和发展。
Objective To investigate the role of primary activation of phosphorylated signal transduction and activator of transcription 3 (p-STAT) and interleukin (IL)-17 expression in the development and progression of breast cancer. The impact on the prognosis. Methods Envision two-step immunohistochemistry method was used to detect the expression of IL-17 and the activation of primary p-STAT3 in 379 cases of breast cancer and matching paraneoplastic 245 cases of breast adenopathy. Their histological types with breast cancer were analyzed. , TNM staging, clinical staging, and prognosis. Statistical analysis: The t-test was used for the measurement data, and the χ2 test was used for the count data. Results 1. The positive expression rate of IL-17 in breast cancer was 95.8%, which was significantly higher than that in breast adenosis 85.3% (χ2=21.363, P<0.001); p-STAT3 with activity in breast cancer The positive rate was 93.6%, which was also significantly higher than the positive rate in breast adenopathy (62.0%) (χ2=97.702, P<0.001). 2. The positive rates of IL-17 and p-STAT3 in breast cancer (including breast invasive ductal carcinoma, breast infiltrating lobular carcinoma, and ductal in situ carcinoma classification) were not correlated with the histological type of breast cancer (χ2= 1.245, P=0.535>0.05). The strong positive rate of IL-17 and pSTAT3 in breast cancer was positively correlated with lymph node metastasis respectively (IL-17: χ2=7.806, P<0.01; p-STAT3: χ2=4.053, P<0.05). In breast cancer tissues, IL-17 expression was positively correlated with p-STAT3 activity (rs=0.136, P<0.01). Conclusion The primary increased activity of p-STAT3 and the highly expressed inflammatory cytokine IL-17 may act synergistically to produce an inflammatory microenvironment and participate in the occurrence and development of breast cancer.