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目的 研究单一HGV GBV C感染者肝组织中该病毒核酸定位及相关抗原表达 ,探讨HGV GBV C感染在肝细胞损伤中的作用。方法 12例单一HGV GBV C感染者经肝穿获取肝组织常规病理诊断 ,采用地高辛标记探针原位杂交法检测病毒RNA ,并用免疫组织化学法检测病毒相关抗原表达。结果 病理诊断急性肝炎 8例 ,慢性肝炎 4例。HGV GBV CNS5抗原检出阳性率为 66.67% ( 8 12 ) ,阳性信号主要位于肝细胞胞浆中 ;HGV GBV CRNA检出阳性率为 58.33% ( 7 12 ) ,阳性信号位于胞浆 ,分布无一定规律 ,阳性细胞与肝细胞变性、淤胆、炎性细胞浸润、细胞坏死程度等并无相关联系。单一HGV GBV C感染者临床表现轻 ,不易被发现。结论 HGV GBV CRNA并不直接损害肝细胞 ;肝细胞中存在HGV GBV C相关抗原表达 ,其编码产物可能作为一种靶抗原 ,诱发免疫病理反应。
Objective To investigate the localization of nucleic acid and the expression of related antigens in the liver tissue of a single HGV-infected GBV C virus and to explore the role of HGV GBV C infection in hepatocellular injury. Methods Twelve patients with single HGV infection of GBV C were routinely diagnosed with liver biopsy. The viral RNA was detected by digoxigenin labeled probe in situ hybridization and the expression of virus associated antigen was detected by immunohistochemistry. Results Pathological diagnosis of acute hepatitis in 8 cases, 4 cases of chronic hepatitis. The positive rate of HGV GBV CNS5 antigen was 66.67% (8 12). The positive signal was mainly located in the cytoplasm of hepatocytes. The positive rate of HGV GBV CRNA was 58.33% (7 12). The positive signal was located in the cytoplasm without any definite distribution Law, positive cells and liver cell degeneration, cholestasis, inflammatory cell infiltration, the degree of cell necrosis and so no correlation. Single HGV GBV C infected patients with mild clinical manifestations. Conclusions HGV GBV CRNA does not directly damage hepatocytes. The expression of HGV GBV C-related antigen exists in hepatocytes, and the encoded product may serve as a target antigen to induce immunopathological responses.