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内毒素对长期透析患者的影响已越来越为人们重视.通过了解复用后血仿膜的超微结构改变,探讨内毒素进入血液的途径、方式及影响,以提高长期透析效果。材料与方法透析机为Gambro。AK-10,每月常规次氯酸钠消毒一次,每次透析后热消毒。水处理系统,反渗机,PVC输水管,常规定期消毒,从各部位取水样每月作细菌培养和鲎试验一次,共12个月。透析器为日本产血仿膜,复用方法为2公斤压力软水冲30分钟后以1%NaOH灌注浸泡12小时,软水冲洗后灌2%福尔马林,24小时后备用。透析液由药材科常规制备。穿刺针为一次性使用。取新透析器一个,复用五次的二个,复用十次的一个,打开外壳后随机抽取纤维丝各三根作纵横切后在扫描电镜下观察膜的改变(电镜观察由北京医科大学电镜室协助)。
The effect of endotoxin on long-term dialysis patients has been paid more and more attention.By understanding the ultrastructural changes of hemodialysis membrane, we explore the ways, methods and effects of endotoxin into blood to improve long-term dialysis. Materials and Methods Dialysis machine for Gambro. AK-10, regular sodium hypochlorite disinfection once a month, after each heat disinfection. Water treatment system, reverse osmosis, PVC pipes, regular regular disinfection, water samples taken from all parts of the monthly bacterial culture and 鲎 test time, a total of 12 months. Dialyzer for Japanese blood production Imitation membrane, reuse method for 2 kg pressure soft water 30 minutes after infusion with 1% NaOH perfusion soak 12 hours, soft water irrigation after irrigation 2% formalin, 24 hours standby. Dialysate prepared by the herbs routine. Puncture needle for one-time use. Take a new dialyzer, reuse five of the two, reuse one of ten times, open the shell and then randomly selected three fibers for longitudinal and transverse cut after scanning electron microscopy to observe changes in the membrane (electron microscopy by the Beijing Medical University, electron microscopy Room assistance).