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目的:探讨联合培养根尖牙乳头干细胞(stem cells from the apical papilla,SCAPs)和人脐静脉血管内皮细胞(human umbilical vein endothelial cells,HUVECs)对牙髓组织血管形成能力的影响。方法:分别使用α-MEM培养基和成骨、成脂、成神经诱导培养基处理SCAPs,采用油红O染色、茜素红染色、βIII-Tubulin免疫荧光染色,分别检测SCAPs的成脂、成骨和神经向分化能力。取单独SCAPs、HUVECs细胞以及联合培养SCAPs和HUVECs细胞进行小管形成实验,分别在3、6、9 h检测各组小管形成的长度、节点数目和结合区面积。采用SPSS16.0软件包对数据进行统计学分析。结果:染色结果显示,实验组SCAPs形成了更多的矿化结节、红色脂滴和神经样细胞。小管形成实验结果表明,联合培养组细胞可以更快地形成类血管状结构,差异显著。结论:SCAPs具有成骨、成脂和神经向诱导分化能力,联合培养SCAPs和HUVECs可以加速血管结构的形成。
OBJECTIVE: To investigate the effects of co-cultured stem cells from the apical papilla (SCAPs) and human umbilical vein endothelial cells (HUVECs) on the angiogenic capacity of dental pulp tissue. Methods: SCAPs were treated with α-MEM and osteogenic, adipogenic and neural inducing medium respectively. The adipocytes were stained with oil red O staining, alizarin red staining and βIII-Tubulin immunofluorescence. Bone and nerve to differentiate. Tubular formation was performed on SCAPs and HUVECs alone and in co-cultures of SCAPs and HUVECs. The length of tubules, the number of nodes and the area of the tubules were detected at 3, 6 and 9 h. SPSS16.0 software package for statistical analysis of the data. Results: The results of staining showed that SCAPs in the experimental group formed more mineralized nodules, red lipid droplets and neuron-like cells. The results of tubule formation experiment showed that the cells in the co-culture group could form the vascular-like structure more rapidly, with significant difference. CONCLUSIONS: SCAPs have osteogenic, adipogenic and neurogenic differentiation potential. Co-culture of SCAPs and HUVECs can accelerate the formation of vascular structures.