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报道了一些影响双醛化被动血凝法检测霍乱肠毒素（ＣＴ）的因素。通过试验认为红细胞的固定方法、致敏的ｐＨ、致敏物的浓度、以及致敏红细胞的浓度均对血凝有影响．用纯化抗ＣＴ－ＰｃＡｂ（ＭｃＡｂ）致敏双醛化羊红细胞作反向被动血凝，可以测出每毫升含２４ｎｇＣＴ．致敏红细胞于４Ｃ保存３个月其血凝滴度无明显变化，纯化抗体在４Ｃ保存４个月，致敏效果未见下降。对二种致敏方法进行比较，铬－免疫球蛋白－鞣酸法和Ｈｏｌｌｉｎｇｅｒ法检测ＣＴ敏感性一致，未经纯化的抗体致敏红细胞，效果较差，不直使用。抗ＣＴ－ＰｃＡｂ（ＭｃＡｂ）致敏羊血球对ＣＴ的检测具有较高的特异性和敏感性。
Several factors affecting the detection of Cholera enterotoxin (CT) by diacondensed passive coagulation were reported. Through experiments that red blood cell fixation method, sensitized pH, the concentration of sensitizers, as well as the concentration of sensitized erythrocytes have an impact on the blood coagulation. Purified anti-CT-PcAb (McAb) sensitized dialdehyde sheep sheep erythrocytes reverse passive coagulation, can be measured per ml with 24ngCT. There was no significant change in the hemagglutination titer of the sensitized erythrocytes at 4C for 3 months. The purified antibody was stored at 4C for 4 months without any decrease in sensitization effect. Comparison of the two sensitization methods, chromium - immunoglobulin - tannin method and Hollinger method consistent with the sensitivity of CT, unpurified antibodies sensitized erythrocytes, the effect is poor, not straightforward use. Anti-CT-PcAb (McAb) sensitized sheep blood cells on CT detection with high specificity and sensitivity.