用牛奶纯化的脂蛋白脂肪酶(LPL),一分子量为56KD,免疫Balb/c小鼠,利用杂交瘤技术,建立了30余株分泌抗朱LPL单克隆抗体的杂交瘤细胞。对其中2E_5、2G_(10)、6D_7、8D_2、7F_4进行特征分析表明:①其抗体类别均为IgG,亚类分别为IgG_1、TgG_(2b)、IgG_(2b)、IgG_1、IgG_1;②抗体效价(细胞培养上清)5×10~(-2)——2×10~(-3);③5种单抗可认为识别三个不同的抗原表位;④Western—blot及Dot—blot结果显示各单抗均能识别纯化的LPL,与人奶均无交叉反应,与牛奶显示不同的结合特性。 A total of more than 30 hybridoma cells secreting anti-Zhu LPL monoclonal antibodies were established by using hybridoma technology with milk-purified lipoprotein lipase (LPL), a molecular weight of 56 kD, immunized Balb / c mice. The characteristic analysis of 2E_5, 2G_ (10), 6D_7, 8D_2, 7F_4 showed that: ①The antibody classes were IgG, the subclasses were IgG_1, TgG_ (2b), IgG_ (2b), IgG_1, IgG_1; (Cell culture supernatant) 5 × 10 -2 - 2 × 10 -3; ③ 5 kinds of monoclonal antibodies can be considered to recognize three different epitopes; ④ Western-blot and Dot-blot results show Each mAb recognizes purified LPL, has no cross-reaction with human milk, and shows different binding properties to milk.