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目的：建立余甘子叶的指纹图谱。方法采用高效液相色谱法测定，乙腈-0.1%磷酸溶液梯度洗脱，流速1.0 ml/min，柱温25℃，紫外检测波长218 nm。结果余甘子叶中有15个共有峰被确定为指纹峰，采用叶中药色谱指纹图谱相似度评价系统A版》计算机软件评价10批余甘子叶指纹图谱的相似性，其结果均>0.9。结论该方法简便，稳定，精密度高，重现性好，可用于余甘子叶的鉴别，作为质量控制方法。“,”Objective To establish a method of fingerprint analysis on Phyllanthus emblica L. by HPLC. Methods The chromatographic finger-prints were obtained by HPLC.The detective wave-length was set at 218 nm;the flow rate was 1.0 ml/min and the column temperature was maintained at 25℃. Results 15 co-possessing peaks were selected as the fingerprint peaks of Phyllanthus emblica L. and good similarities with correlation coefficients higher than 0.9 were found in fingerprints between the different samples of Phyllanthus emblica L. and the standard fingerprint. Conclusion The method with good reproducibility is simple and accurate,it can be used as an effective quality control method for Phyllanthus emblica L.