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针对中国海南地区芒果蒂腐病致病菌可可球二孢对多菌灵的抗性水平及菌株的适合度进行了研究。采用区分剂量法,测定了2016年从海南省芒果园采集、分离的90株可可球二孢对多菌灵的敏感性,并对抗性及敏感菌株在菌丝生长、致病力、渗透压及相对渗率等方面进行了比较。结果表明:海南地区芒果可可球二孢对多菌灵的抗性频率为65.56%,且以高抗菌株为主(8个中抗菌株,51个高抗菌株);该病菌对芒果危害甚大,其中具强致病力的菌株达65株,占72.22%。高抗菌株的菌丝生长优于敏感菌株,存在极显著差异(P<0.01);抗性菌株的致病力与敏感菌株无显著差异;敏感菌株的相对渗率高于抗性菌株,表明敏感菌株细胞膜的透性较高,胞内电解质渗出较多。研究表明,海南地区芒果可可球二孢对多菌灵的抗性水平较高,且抗性菌株适合度高于敏感菌株,易形成优势群体。
In order to study the resistance level of Carbendazim to the pathogen of Pedicola rot in mango fruit in Hainan, China and the suitability of the strains. Distinguishing dose method was used to determine the sensitivity of 90 strains of Cocosidia sp. Collected from Hainan mango orchards in 2016 to the growth of mycelia. The resistance and susceptibility of the strains to mycelium growth, pathogenicity, osmotic pressure and Relative permeability and other aspects were compared. The results showed that the frequency of resistance to carbendazim in Hainan was 65.56%, and the main strains were high antibacterial strains (8 medium antibacterial strains and 51 high antibacterial strains) Among them, 65 strains were highly virulent, accounting for 72.22%. The mycelial growth of high antibacterial strains was superior to that of the sensitive ones (P <0.01). The virulence of the resistant strains was not significantly different from that of the susceptible ones. The relative permeability of the susceptible strains was higher than that of the resistant ones, indicating the sensitivity The membrane permeability of the strain is higher, and the intracellular electrolytes exude more. The results showed that the resistance of Coccinea mongolicus to carbendazim in Hainan was higher, and the fitness of resistant strains was higher than that of susceptible strains, which led to the formation of dominant populations.