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以两份普通野生稻核心种质资源DP15和DP30为供体、9311为受体构建染色体片段代换系鉴定苗期耐冷性QTL;利用苗期耐冷性最强的1个代换系构建QTL作图群体,用SSR标记对其主效QTL进行定位。研究结果表明,两个抗源DP15和DP30所含的苗期耐冷性QTL的数量、位点及耐冷性效应均存在明显的差异。在基本上覆盖两个亲本全基因组的230份BC4F2代换系中共发现19个苗期耐冷性QTL,分布在水稻12条染色体上,第3和第8染色体上有比较密集的苗期耐冷性QTL分布。这19个分布于全基因组的苗期耐冷性QTL被分别分离到不同的野生稻染色体片段代换系里,效应最小的微效QTL位点所在的代换系在苗期耐冷性鉴定中的活苗率仅为8%,而效应最大的主效QTL位点所在代换系的活苗率达到74%。这个主效QTLqSCT-3-1被定位在第3染色体着丝点附近长臂上的RM15031-RM3400区间,距离最近的标记RM15040、RM1164的遗传距离为1.8 c M。
Two QTL for core collection of common wild rice (Oryza sativa L.) germplasm DP15 and DP30 and 9311 as the receptor were used to identify QTLs for cold tolerance in seedling stage. QTLs were constructed by using one substitution line with the strongest cold tolerance at seedling stage In the population, the QTLs for major traits were mapped with SSR markers. The results showed that there were significant differences in the number of cold tolerance QTLs, loci and cold tolerance among the two DP15 and DP30 resistant cultivars. A total of 19 QTLs for cold-tolerance at seedling stage were found in 230 BC4F2 substitution lines that basically covered the whole genome of two parents. The QTLs were located on 12 chromosomes of rice, and the QTLs for cold tolerance at seedling stage on chromosomes 3 and 8 distributed. The QTLs for cold tolerance at the seedling stage of 19 isolates were isolated from different genomes of the chromosome segment substitution lines of O. sativa. The substitution lines with the least effect microleakage QTL loci were identified as cold tolerance in seedling stage The seedling rate was only 8%, but the viable rate of the substitution line where the major effect QTL locus was reached was 74%. This major QTL QSCT-3-1 was mapped on the RM15031-RM3400 interval on the long arm near the centromere on chromosome 3, with the closest marker at RM15040 and RM1164 at a genetic distance of 1.8 cM.