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目的 研究外源性化学物代谢酶基因多态性与焦炉作业工人外周血淋巴细胞染色体损伤的关系。方法 选取 14 9名焦炉作业工人和 2 4名非职业多环芳烃 (PAH)暴露人员作为研究对象 ,测定其尿中 1 羟基芘浓度来反映PAH暴露的内剂量 ;对照组的外周血淋巴细胞微核水平的上 4分位数 (6‰ )作为判断个体染色体损伤阳性的界值 ;分析CYP1A1、GSTM1、GSTT1、GSTP1、CYP2E1、NQO1、NAT2和mEH基因的多态性 ;使用多元logistic回归方程校正职业暴露情况、年龄、性别、吸烟和饮酒状况因素 ,计算不同基因型工人发生染色体损伤阳性的OR值 ,并探讨基因间的交互作用。结果 调整了 173名研究对象的职业暴露、年龄、性别、吸烟和饮酒状况后 ,GSTM 1缺失基因型个体染色体损伤危险度显著性增加 (调整OR =2 0 1,95 %CI =1 0 3～ 3 91) ;与NQO1基因P187S位点野生型纯合子个体比较 ,变异型纯合子个体染色体损伤危险度显著性增加 (调整OR =3 18,95 %CI=1 18～ 8 6 2 ) ;与mEH基因H113Y位点野生型纯合子个体比较 ,变异型纯合子个体染色体损伤危险度显著性降低 (调整OR =0 4 0 ,95 %CI =0 19～ 0 88) ;未发现其他基因的遗传变异与研究对象外周血淋巴细胞染色体损伤危险度的显著关联。此外 ,还发现GSTM1、NQO1基因P187S位点和mEH基?
Objective To study the relationship between polymorphisms of exogenous chemical metabolic enzymes and chromosomal damage of peripheral blood lymphocytes in coke oven workers. Methods 14 9 workers exposed to coke oven and 24 persons exposed to PAH were enrolled in this study. The concentration of 1-hydroxy-pyrene in urine was measured to reflect the internal dose of PAH exposure. The control group’s peripheral blood lymphocytes The upper 4 quantiles of micronucleus (6 ‰) were used as the cutoff for judging the chromosome damage of individuals. The polymorphisms of CYP1A1, GSTM1, GSTT1, GSTP1, CYP2E1, NQO1, NAT2 and mEH genes were analyzed. Multivariate logistic regression equation Correcting occupational exposure, age, sex, smoking and drinking status factors, calculate the OR value of chromosomal damage positive for different genotype workers, and explore the inter-gene interaction. Results After adjusted for occupational exposure, age, sex, smoking and alcohol consumption of 173 subjects, the risk of chromosomal damage was significantly increased in individuals with GSTM1 deletion genotype (adjusted OR = 20,95% CI = 103 ~ The risk of chromosomal damage was significantly increased in individuals with homozygous homozygotes (adjusted OR = 3 18, 95% CI = 18-1862) compared with individuals homozygous for P187S in NQO1; Genotype H113Y wild-type homozygous individuals compared homozygous mutant homozygous individuals significantly reduce the risk of chromosomal damage (adjusted OR = 0 40, 95% CI = 0 19 ~ 0 88); found no other genetic variation and Significant associations between the risk of chromosomal damage in peripheral blood lymphocytes were studied. In addition, GSTM1, NQO1 gene P187S site and mEH group were also found?