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目的研究持续性心房颤动(房颤)对自主神经的影响。方法快速起搏制备持续性房颤犬模型。停止起搏后待转复为窦性心律,评价其心率变异(HRV)的波动。然后测定左、右心耳,左、右心房游离壁和房间隔心肌中去甲肾上腺素的含量。同时研究心肌中神经生长因子蛋白和β_1受体的表达。并与正常犬对照。结果持续性房颤形成后,交感神经张力增高。长期房颤组(1682±362)ms~2和短期房颤组(1247±219)ms~2的低频成分(LF)高于正常对照组(798±154)ms~2(P<0.01)。而长期房颤组(232±75)ms~2和短期房颤组(310±165)ms~2高频成分(HF)低于正常对照组(1041±195)ms~2(P<0.01)。房颤犬正常 RR 间期标准差(SDNN)和 HRV 三角指数低于正常对照组(P<0.01)。房颤犬心房肌交感神经递质含量增多,心房各部位的去甲。肾上腺素含量均高于对照组的相应部位(P<0.01)。而且心房不同部位的递质含量存在变异,差异有统计学意义(P<0.01)。房颤组心房肌神经生长因子蛋白表达增多,β_1受体表达下调。结论持续性房颤造成心脏交感神经张力增高,心房肌去甲肾上腺素不均一积聚。这种变化可能是通过 NGF 介导的。
Objective To study the effect of persistent atrial fibrillation (AF) on autonomic nervous system. Methods Fast paced preparation of persistent atrial fibrillation dog model. After stopping pacing to be converted to sinus rhythm, to assess the heart rate variability (HRV) fluctuations. The content of norepinephrine in left and right atrial appendage, left and right atrium free wall and atrial septal myocardium was then determined. At the same time study the expression of nerve growth factor protein and β 1 receptor in myocardium. And with normal dogs control. Results After the formation of persistent atrial fibrillation, sympathetic tone increased. LF in long-term atrial fibrillation group (1682 ± 362) ms ~ 2 and short-term atrial fibrillation group (1247 ± 219) ms ~ 2 was higher than that in normal control group (798 ± 154) ms ~ 2 (P <0.01). (HF) was lower than that of the normal control (1041 ± 195) ms ~ 2 (P <0.01) in long-term atrial fibrillation group (232 ± 75) ms ~ 2 and short atrial fibrillation group (310 ± 165) . The normal standard deviation (SDNN) and HRV trigone index of atrial fibrillation dogs were lower than that of the normal control group (P <0.01). Atrial fibrillation atrial sympathetic neurotransmitter content increased, all parts of the atrium. Epinephrine levels were higher than the corresponding parts of the control group (P <0.01). Moreover, the contents of neurotransmitters in different parts of the atria were different, the difference was statistically significant (P <0.01). Atrial fibrillation increased atrial nerve growth factor protein expression, β 1 receptor expression was down. CONCLUSION: Persistent atrial fibrillation results in increased sympathetic tone in the heart and non-uniform accumulation of norepinephrine in the atria. This change may be mediated by NGF.