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目的测定不同品种、不同产地怀地黄中桃叶珊瑚苷的含量,初步对怀地黄种质资源进行评价。方法采用HPLC,Dikma Diamonsil C18色谱柱(4.6 mm×250 mm,0.5μm),流动相为甲醇-水(5.5∶94.5),检测波长为206 nm。结果桃叶珊瑚苷在0.053 5~1.284μg间线性关系良好,回归方程为Y=539 696X+3 581.6(r=0.999 9),回收率分别为99.75%(鲜地黄)和99.61%(生地黄),RSD分别为2.86%和2.15%(n=6);不同品种、不同产地怀地黄中桃叶珊瑚苷含量不同。结论本法操作简单、灵敏、稳定,适用于怀地黄中桃叶珊瑚苷的含量测定。
Objective To determine the content of aucubin in the different breeds of Huai Huang Huang, and to evaluate the germplasm resources of Rehmannia glutinosa. Methods HPLC was used on a Dikma Diamonsil C18 column (4.6 mm × 250 mm, 0.5 μm). The mobile phase was methanol-water (5.5:94.5) and the detection wavelength was 206 nm. Results Aucubin showed a good linear relationship between 0.053 5 and 1.284 μg. The regression equation was Y = 539 696X + 3881.6 (r = 0.999 9). The recoveries were 99.75% (fresh yellow) and 99.61% , RSD were 2.86% and 2.15% respectively (n = 6). The content of aucubin in different breeds and different areas of Huai Huang was different. Conclusion This method is simple, sensitive and stable, suitable for the determination of aucubin in Rehmanniae.