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目的探讨信号素4D(Sema4D)及受体丛状蛋白B1(Plexin-B1)在氟性骨损伤发生中的作用。方法 SPF级SD大鼠48只,随机分为1个对照组和3个染氟组,采用氟化钠(NaF)溶液饮水染毒,3个染氟组NaF染毒浓度分别为1.6、16和32 mg/kg。在染氟第0天、第15天、第30天和第90天时,进行大鼠尾动脉取血,分别测定各时段血氟(微量电极法)、ALP(全自动生化分析仪法)和Sema4D/Plexin-B1(ELISA法)。结果染氟30 d后,3个染氟剂量组大鼠均发生氟斑牙,表现为下切牙色泽度差,表面棕、白色带增宽,横纹不规则甚至消失,并可见牙齿缺损。大鼠血氟与ALP随染毒剂量增加和时间延长呈升高趋势,与对照组比较,差异有统计学意义(P<0.01)。大鼠Sema4D含量在染氟第15天开始降低,组间比较,差异有统计学意义(P<0.05)。染氟第30天,高剂量组(12.20±0.73 ng/1L)和中剂量组(13.70±0.68 ng/L)大鼠Sema4D含量下降明显,与对照组(19.20±0.68 ng/L)比较差异有统计学意义(P<0.01)。染氟第90天,高、中、低剂量组大鼠Sema4D含量分别为(11.14±0.66)、(11.73±0.68)和(13.72±0.71)ng/L,与对照组(19.87±0.69 ng/L)比较明显降低(P<0.01)。大鼠Plexin-B1含量也随着染氟时间的延长而有所下降,且高、中2个剂量组大鼠Plexin-B1含量下降较为明显,与对照组比较,差异有统计学意义(P<0.01)。结论染氟组大鼠血清中Sema4D和Plexin-B1表达量明显降低,大鼠氟性骨损伤的发生可能与Sema4D/Plexin-B1表达水平有关。
Objective To investigate the role of Sema4D and Plexin-B1 in the pathogenesis of fluorosis bone injury. Methods Forty eight Sprague-Dawley (SD) SD rats were randomly divided into 1 control group and 3 fluoride-exposed groups. Sodium fluoride (NaF) solution was used to infect water. The concentrations of NaF in 3 fluoride groups were 1.6, 16 32 mg / kg. Blood was taken from the caudal artery of rats at day 0, day 15, day 30, and day 90 of staining for fluorine, and blood levels of fluoride (microelectrode), ALP (automatic biochemical analyzer) and Sema4D / Plexin-B1 (ELISA method). Results After 30 days of exposure to fluoride, dental fluorosis occurred in all the three fluoride-exposed rats. The results showed that the lower incisors had poor color, widened brown and white bands, irregular or even horizontal stripes, and visible tooth defects. Blood fluoride and ALP in rats increased with the increase of dose and prolongation of time, compared with the control group, the difference was statistically significant (P <0.01). The level of Sema4D in the rats began to decrease on the 15th day after fluoride exposure. There was significant difference between the two groups (P <0.05). The level of Sema4D in the high dose group (12.20 ± 0.73 ng / L) and the medium dose group (13.70 ± 0.68 ng / L) on day 30 was significantly lower than that of the control group (19.20 ± 0.68 ng / L) Statistical significance (P <0.01). At the 90th day after exposure to fluoride, the levels of Sema4D in the high, middle and low dose groups were (11.14 ± 0.66), (11.73 ± 0.68) and (13.72 ± 0.71) ng / L, respectively, and those in the control group were 19.87 ± 0.69 ng / L ) Was significantly lower (P <0.01). The content of Plexin-B1 in rats also decreased with the prolongation of fluoride exposure, and the levels of Plexin-B1 in high and middle dose groups decreased more obviously than those in control group (P < 0.01). Conclusion The expression of Sema4D and Plexin-B1 in serum of rats with fluorosis is obviously decreased. The occurrence of fluorosis bone injury may be related to the expression of Sema4D / Plexin-B1.