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DTX4(Deltex 4 homolog)蛋白属于Deltex家族成员;Deltex家族是Notch信号通路的调节因子.已知Notch信号通路在成肌分化中发挥重要作用.然而,DTX4是否参与调控肌肉发育尚未有报道.本研究探索DTX4对成肌分化的影响及作用机制.实时定量PCR和蛋白质印迹分析揭示,伴随小鼠C2C12成肌细胞(myoblast)分化为肌管(myotube)过程,成肌分化标志蛋白肌球蛋白重链(myosin heavy-chain,My HC)、肌细胞生成素(myogenin)表达逐渐升高,DTX4 mRNA及蛋白质表达水平也逐渐升高.通过顺序专一的siRNA敲减DTX4表达后,C2C12成肌细胞肌管面积和肌管融合指数明显减少;My HC、肌细胞生成素蛋白表达水平明显降低;但ERK信号通路未见明显变化.上述结果表明,敲减DTX4表达抑制C2C12细胞成肌分化.我们的结果提示,DTX4可能参与C2C12细胞成肌分化.
The Deltex 4 homolog protein is a member of the Deltex family and the Deltex family is a regulator of the Notch signaling pathway.It is known that Notch signaling pathway plays an important role in myogenic differentiation.However, whether DTX4 is involved in the regulation of muscle development has not been reported.In this study To investigate the effect of DTX4 on myogenic differentiation and its mechanism of action.Real-time quantitative PCR and Western blot analysis revealed that myoblast differentiation of myoblast mouse myotube, myo-differentiation marker protein myosin heavy chain myosin heavy-chain (My HC), myogenin expression and DTX4 mRNA and protein expression gradually increased.Through specific knockdown of DTX4 by specific siRNA, C2C12 myoblast The area of myotubes and the fusion index of myotubes decreased significantly; My HC, myogenin protein expression decreased significantly, but there was no significant change of ERK signal pathway.The above results show that knockdown of DTX4 expression inhibits the myogenic differentiation of C2C12 cells.Our results Tip, DTX4 may be involved in C2C12 cells myogenic differentiation.