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mRNA差异显示PCR技术是近年来出现的一种新的显示mRNA差异表达的方法,它的应用将基因表达的研究提高到了一个新的水平。本文就该技术的基本原理与方法、优缺点及近年来在方法学上的研究进展作一综述,并对该技术在遗传毒理学研究中的应用前景予以初步探讨。
mRNA Differential Display PCR is a new method of displaying differential expression of mRNA in recent years. Its application has raised the study of gene expression to a new level. In this paper, the basic principles and methods, advantages and disadvantages of the technology and the progress in methodology in recent years are reviewed, and the application prospect of the technology in genotoxicity research is discussed.