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目的优化平贝母总生物碱提取工艺,分析不同新炮制品的总生物碱变化情况,为其开发利用提供理论依据。方法参考前人的研究成果,采用正交实验法优化平贝母总生物碱提取工艺;采用传统中药的炮制方法(醋炙、酒炙、姜炙、蜜炙)加工平贝母新产品。结果 75%乙醇提取2次,每次提取时间2h,总生物碱得率最高;与传统加工品中的总生物碱含量(0.191%)相比,醋炙、酒炙分别为0.242%、0.204%;姜炙、蜜炙分别为0.186%、0.167%。结论平贝母中总生物碱的最佳提取工艺为:75%乙醇提取2次,每次提取时间2h。醋炙、酒炙能有效地提高平贝母的有效成分含量;姜炙、蜜炙则相反。
OBJECTIVE To optimize the extraction process of total alkaloids from Fritillaria ussuriensis and analyze the changes of total alkaloids in different new processed products, providing a theoretical basis for its development and utilization. Methods According to the results of previous studies, the orthogonal experiment was used to optimize the extraction process of total alkaloids of Fritillaria ussuriensis. The new products of Fritillaria hirsutum were processed by traditional Chinese medicine methods (vinegar, wine, sunburn, honeyburn). Results The extraction rate of total alkaloids was the highest with 75% ethanol extraction twice per extraction time. Compared with the total alkaloid content (0.191%) in the traditional processed products, vinegar and wine broilers were 0.242% and 0.204% ; Ginger Sunburn, honey Sunburn were 0.186%, 0.167%. Conclusion The optimum extraction process of total alkaloids in Fritillaria thunbergii is: extraction with 75% ethanol twice, extraction time 2h. Vinegar Sunburn, wine Sunburn can effectively improve the Fritillaria the effective content; ginger Sunburn, honey Sunburn is the opposite.