论文部分内容阅读
目的:建立测定生长激素(GH)在体生物活性的方法.方法:以去垂体大鼠体重增长(BWG)和胫骨骺软骨板宽度(TEW)为指标,观察动物性别、给药途径、次数和周期不同对效应的影响;同时进行4dBWG,6dBWG和6dTEW法,测定GH的效价(平行线3×3设计).结果:♀和♂sc和im给药以及每日给药1次和2次的BWG和TEW差异无显著意义.给药6d比给药4d引起较大的BWG和TEW(P<005).4dBWG法和6dBWG法在0020-0500IU·d-1有较好的λ值(00660和01747)和r值(09000和09237);4dBWG,6dBWG和6dTEW法测得rhGH的效价为46132,39829和48023IU/amp.6dBWG法有较小的λ值和较低的ARFL值.结论:可在同一组去垂体大鼠体内同时用4dBWG,6dBWG和6dTEW法测GH活性,以6dBWG法较好.
Objective: To establish a method for determining the in vivo biological activity of growth hormone (GH). Methods: The effects of different sex, route of administration, number of times and cycles on the effect were observed by weight gain (BWG) and epiphyseal cartilage plate width (TEW) of the pituitary gland. At the same time, 4dBWG and 6dBWG And 6 dTEW method, determination of GH titer (parallel lines 3 × 3 design). RESULTS: There was no significant difference in BWG and TEW between ♀ and ♂ sc and im administration, and once and twice daily dosing. Administration of 6d resulted in greater BWG and TEW than 4d administration (P <005). 4-dBWG method and 6-dBWG method have better λ values (00660 and 01747) and r values (09000 and 09237) at 020-0500 IU · d-1; 4 The titers of rhGH measured by dBWG, 6dBWG and 6dTEW methods were 4.132, 3.9829 and 4.8023IU / amp. 6 dBWG method has a smaller λ value and lower ARFL value. Conclusion: In the same group of pituitary rats in vivo at the same time with 4 dBWG, 6 dBWG and 6 dTEW method to measure GH activity, 6 dBWG method is better.