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采用AlltimaODS C18柱,以0.02mol/L醋酸铵溶液(pH6.5)—甲醇(80:20,V/V)作流动相进行,流速1.0mL/min,用二极管阵列检测器在280nm下进行检测,建立液相色谱—二极管阵列检测法测定酵母浸膏中水解和游离色氨酸的方法。结果显示,方法线性范围为2.5～20.0μg/mL(r=0 9999),检测限0.3μg/mL,相对标准偏差为1.31%(n=6),回收率为96.4±1.99%(n=3)。
AlltimaODS C18 column was used and the mobile phase consisted of 0.02mol / L ammonium acetate solution (pH6.5) -methanol (80:20, V / V) was used as the mobile phase at a flow rate of 1.0mL / min and detected with a diode array detector at 280nm , The establishment of liquid chromatography - diode array detection method of yeast extract hydrolysis and free tryptophan. The results showed that the linear range was 2.5-20.0μg / mL (r = 0 9999), the detection limit was 0.3μg / mL, the relative standard deviation was 1.31% (n = 6) and the recovery was 96.4 ± 1.99% ).