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试验在表面活性剂溴代十六烷基吡啶和酸性Tris-盐酸缓冲介质存在下,刚果红与维生素B1反应的瑞利散射光谱。体系的最大瑞利散射峰位于372 nm波长处,体系的瑞利散射增强程度(ΔIRLS)与0.06~0.42 mg/L范围内维生素B1的质量浓度呈良好的线性关系,该方法的检出限为0.002 8 mg/L。还对反应条件及瑞利散射光谱特征进行了研究。该方法用于奶粉中维生素B1的测定,回收率为98.7%~102%,相对标准偏差(n=6)为1.5%~1.8%。
Rayleigh scattering spectra of Congo red and vitamin B1 were tested in the presence of surfactants such as cetyl pyridinium bromide and acidic Tris-HCl buffer. The maximum Rayleigh scattering peak of the system is located at the wavelength of 372 nm. The Rayleigh scattering enhancement (ΔIRLS) of the system shows a good linear relationship with the concentration of vitamin B1 in the range of 0.06 ~ 0.42 mg / L. The detection limit of this method is 0.002 8 mg / L. The reaction conditions and Rayleigh scattering spectral characteristics were also studied. The method was applied to the determination of vitamin B1 in milk powder with the recoveries of 98.7% ~ 102% and the relative standard deviations (n = 6) of 1.5% ~ 1.8%.