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目的探讨不同浓度塞来昔布对乳腺癌细胞MCF-7增殖、侵袭的影响以及抗肿瘤作用。方法通过MTT法检测不同浓度的塞来昔布(0、20、40、60、80和100μmol/L)对转染COX-2人乳腺癌细胞株MCF-7增殖的影响。通过细胞游走浸润法检测不同浓度的塞来昔布(60、100μmol/L)对人乳腺癌细胞株MCF-7游走能力的影响。结果 20、40、60、80、100μmol/L塞来昔布作用于MCF-7细胞72 h,其生存率分别达(67.3±5.2)%、(53.8±3.8)%、(45.6±2.9)%、(39.4±3.1)%、(31.7±2.7)%。塞来昔布各剂量组对MCF-7细胞增殖均具有抑制作用,生存率随药物浓度的增加而减低(P<0.01)。60、100μmol/L塞来昔布作用于MCF-7细胞24 h,侵袭细胞数分别达80.6、54.2。塞来昔布各剂量组对MCF-7细胞游走均具有抑制作用,侵袭游走能力随药物浓度的增加而降低(P<0.01)。结论塞来昔布有抑制乳腺癌细胞MCF-7增殖的作用,且呈剂量依赖性。塞来昔布有抑制乳腺癌细胞MCF-7游走的作用,且呈剂量依赖性。
Objective To investigate the effects of different concentrations of celecoxib on the proliferation and invasion of breast cancer cell line MCF-7 and the antitumor effects. Methods The effects of different concentrations of celecoxib (0, 20, 40, 60, 80 and 100 μmol / L) on the proliferation of human breast cancer cell line MCF-7 transfected with COX-2 were detected by MTT assay. The effect of different concentrations of celecoxib (60,100μmol / L) on the migration ability of human breast cancer cell line MCF-7 was detected by cell migration infiltration method. Results The survival rates of MCF-7 cells treated with 20, 40, 60, 80, 100μmol / L celecoxib for 72 h were 67.3 ± 5.2%, 53.8 ± 3.8% and 45.6 ± 2.9% , (39.4 ± 3.1)% and (31.7 ± 2.7)%, respectively. Celecoxib inhibited the proliferation of MCF-7 cells and the survival rate decreased with the increase of drug concentration (P <0.01). MCF-7 cells were treated with 60,100μmol / L celecoxib for 24 h, the number of invasive cells was 80.6 and 54.2 respectively. Each dose of celecoxib inhibited the migration of MCF-7 cells, and the invasion and migration ability decreased with the increase of drug concentration (P <0.01). Conclusion Celecoxib can inhibit the proliferation of breast cancer cells MCF-7 in a dose-dependent manner. Celecoxib inhibited the MCF-7 migration in breast cancer cells in a dose-dependent manner.