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目的 :探讨中药骨康对绝经后骨质疏松大鼠模型血清骨钙素的影响。方法 :采用雌性 SD大鼠 ,运用随机对照分组的原则 ,分别分为正常对照组、模型组、中药骨康组及尼尔雌醇组 ,去双侧卵巢后造绝经后骨质疏松大鼠模型 ,造模三个月后 ,分别采用生理盐水、中药骨康及尼尔雌醇对其进行灌胃 ,灌胃三个月后采用摘眼球取血 ,离心分离血清 ,放免分析法测定其血清骨钙素含量 ,并行离体右胫骨双能 X线扫描。结果 :中药骨康组大鼠血清骨钙素水平明显高于模型组及尼尔雌醇组 ,两者相比有显著性差异 ,P<0 .0 5 ;骨康治疗组大鼠离体骨密度显著高于模型组 ,两者相比 P<0 .0 5。结论 :中药骨康能显著提高模型大鼠血清骨钙素水平 ,并能显著提高其离体骨骨密度 ,表明其具有促进去势模型大鼠骨形成的作用
Objective: To explore the effect of Chinese medicine GuKang on serum osteocalcin in postmenopausal rats with osteoporosis. METHODS: Female Sprague-Dawley rats were divided into normal control group, model group, traditional Chinese medicine Gukang group and nilestriol group according to the principle of randomized controlled group. The post-ovarian model of postmenopausal osteoporosis was established in rats. After three months of modeling, normal saline, traditional Chinese medicine, Gukang, and nilestriol were used to gavage them. After three months of gavage, blood was collected using eyeballs, serum was separated by centrifugation, and serum was determined by radioimmunoassay. Calbin content, parallel dual-energy X-ray scan of the right iliac bone. RESULTS: The serum osteocalcin level in the Gukang group was significantly higher than that in the model group and the nilestriol group. There was a significant difference between the two groups. P<0.05; Gukang treatment group isolated rat bone The density was significantly higher than that of the model group, which was P<0.05. Conclusion : Chinese medicine Gukang can significantly increase serum osteocalcin level in model rats and can significantly increase the bone density of isolated bone, indicating that it can promote bone formation in ovariectomized rat models.