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目的探讨血浆(1,3)-β-D葡聚糖(1,3 beta-D-glucan, BDG)和半乳甘露聚糖(galactomannan, GM)抗原联合检测对侵袭性真菌感染(invasive fungal infections, IFI)的临床诊断价值，为临床及时合理用药提供依据。方法根据中华医学会《重症患者侵袭性真菌感染诊断与治疗指南(2007)》，将215例临床怀疑真菌感染的患者分为侵袭性真菌病组72例和非感染组143例。采集患者外周血并应用MB-80微生物动态快速检测系统和酶联免疫吸附法定量检测血浆(1,3)-β-D葡聚糖及半乳甘露聚糖含量，将检测结果进行统计学分析。结果 G试验和GM试验的敏感性、特异性、阳性预测值和阴性预测值分别是55.56%和48.61%，86.01%和79.02%，66.67%和53.85%，79.53%和75.33%。 G/GM试验的敏感性、特异性、阳性预测值和阴性预测值分别是76.39%，75.52%，61.11%，86.40%。经统计学分析，血浆(1,3)-β-D葡聚糖和半乳甘露聚糖抗原联合检测可提高临床诊断的灵敏度。结论 G试验和GM试验对IFI早期诊断有一定诊断价值，而两者联合检测大大提高了灵敏度，对IFI的诊断更有价值。“,”Objective To evaluate the clinical diagnostic value of plasma G (1,3-β-D- glucan)/GM (galactomannan) combined detection in diagnosis of invasive fungal infection(IFI). And than it can provide the basis for clinical rational use of drugs in a timely manner. Methods There were 215 patients of the clinical suspicion of IFI, then these were divided into 72 IFI and 143 no-IFI. The concentration of plasma 1,3-β-D-glucan and galactomannan was detected by MB-80 Microbiology Kinetic Rapid Detection System and ELISA. Results The sensitivity, specificity, a positive predictive value(PPV) and a negative predictive value( NPV) were 55.56%, 86.01%, 66.67%, 79.53%in G test, and 48.61%, 79.02%, 53.85%, 75.33%in GM test. But the sensitivity and specificity of G/GM test were 76.39%and 75.52%, with a positive predictive value (PPV) of 61.11%and a negative predictive value ( NPV) of 86.40%. Conclusion The G test and GM test were both useful in diagnosis of IFI. The bet er sensitivity might be acquired by combining these two tests. G/GM test is an early and rapid method for diagnosis of IFI.