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对一些疱疹病毒感染,如Kaposi水痘样疹、原发性或继发性免疫缺陷、临产的妊娠妇女、新生儿、护理重危病人的医护人员都必需及时正确地作出诊断。1947年Tzanck发现于皮损中找到多核巨细胞可用来诊断单纯疱疹、水痘、带状疱疹等疾病。目前诊断上述疾病的方法很多。Tzanck法虽迅速简单,但尚未普遍采用。本文对30例单纯疱疹皮肤感染的32处皮损的病毒分离和Tzanck法诊断可靠性作了比较。13例为初发性皮损,17例为复发性。皮损部位16例在唇部,11例在生殖器,其余在手指、手背和眼睑。方法:用酒精擦拭皮损,待干燥后,用手术刀挑破水疱或刮去痂皮,然后用消毒棉签按擦皮损基部数次,接种在病毒转运溶液中,快速冷冻于-80℃中或接种于
Some herpes virus infections, such as Kaposi chickenpox, primary or secondary immunodeficiency, labor in pregnant women, newborns, nursing care of critically ill patients must be promptly and correctly diagnosed. In 1947 Tzanck found that multinucleated giant cells found in lesions can be used to diagnose herpes simplex, chickenpox, shingles and other diseases. There are many ways to diagnose the above diseases. Although the Tzanck method is quick and easy, it has not been widely adopted. In this paper, 30 cases of herpes simplex skin infections in 32 lesions of the virus isolation and Tzanck method to compare the reliability of diagnosis. 13 cases of primary lesions, 17 cases of recurrent. 16 cases of lesions in the lip, 11 cases of genital, the rest in the fingers, back and eyelids. Methods: Wipe the skin with alcohol. After drying, scalpel or scraping the scab with a scalpel, then rub the scab with a sterile cotton swab for several times and inoculate it in the virus transport solution and rapidly freeze at -80 ℃ Or inoculated to