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目的:探讨乙型肝炎患者血清中HBeAg定量结果与乙型肝炎病毒核酸(HBV-DNA)的检测结果在临床上的意义。方法:本院住院及门诊HBV感染患者248例,抽取静脉血,采用1235全自动时间分辨荧光免疫分析仪和PE5700全自动荧光PCR仪对这248例标本分别进行检测。结果:乙型肝炎患者定量测定e抗原(HBeAg)阳性者其HBV-DNA阳性率远大于HBeAg阴性者(P<0.05);HBsAg及HBeAg2项阳性模式HBeAg定量结果显著大于HBsAg、HBeAg、HBcAb阳性模式(P<0.05),其HBV-DNA波动范围也大于HBsAg、HBeAg、HBcAb阳性模式。结论:HBeAg定量结果能较好地反映乙型肝炎患者体内HBV-DNA的含量,并对患者病情进展和诊治有一定应用价值。
Objective: To investigate the clinical significance of the detection results of serum HBeAg and hepatitis B virus (HBV) DNA in patients with hepatitis B virus. Methods: 248 inpatients and outpatients with HBV infection were enrolled in this study. Venous blood was collected and the 248 samples were detected by 1235 automatic time-resolved fluorescence immunoassay analyzer and PE5700 automatic fluorescence PCR machine. Results: The positive rate of HBV-DNA in HBeAg positive patients was much higher than that in HBeAg negative patients (P <0.05). The positive HBeAg and HBeAg positive HBeAg positive rates were significantly higher than those of HBsAg, HBeAg and HBcAb (P <0.05). The range of HBV-DNA fluctuation was also greater than that of HBsAg, HBeAg and HBcAb positive models. Conclusion: The quantitative results of HBeAg can better reflect the content of HBV-DNA in patients with hepatitis B, and have certain value in the progression of disease and diagnosis and treatment.