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为探索 p15 INK4B基因在CpG岛高甲基化对白血病发病机制的重要作用 ,应用敏感的甲基化特异的MSP PCR的测定方法 ,测定了 p15 INK4B基因在急性粒细胞白血病 (AML)和慢性粒细胞白血病 (CML)中甲基化的表达变化。研究结果表明 ,p15 INK4B基因操纵区在AML和CML中的甲基化发生率分别为 83.9% (2 6/ 31)和 0 % (0 / 2 8)。结论提示 :甲基化是p15 INK4B基因在AML中主要的失活方式之一 ,并可在病程进展中出现甲基化 ,使病情加重 ;在CML中无甲基化的发生 ,说明p15 INK4B基因的功能在CML中可能是完整的
To explore the role of p15 INK4B gene hypermethylation on the pathogenesis of leukemia in CpG islands, a sensitive methylation-specific MSP PCR assay was used to determine the p15 INK4B gene in acute myeloid leukemia (AML) and chronic myelogenous leukemia. Changes in expression of methylation in (CML). The results showed that the methylation rates of the p15 INK4B gene manipulation region in AML and CML were 83.9% (26/31) and 0% (0/28), respectively. Conclusions: Methylation is one of the major inactivation modes of p15 INK4B gene in AML, and methylation may occur during the progression of the disease, which makes the disease worse. There is no methylation in CML, indicating p15 INK4B gene. Functionality may be complete in CML