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目的探讨一个科室多台血细胞分析仪主要项目的准确性和可比性的保证措施。方法依据美国临床实验室标准化委员会制定的关于用患者样本进行对比和偏差评估方案,对3台血细胞分析仪检测结果进行分析及时发现仪器间系统误差。结果 3台血细胞分析仪上WBC、RBC、Hct、Hb、Plt五个参数在的CV值非常接近,CV值都小于5.00%;单核细胞百分数高值相对偏差分别为-24.10%和-20.90%,中值与低值也都接近-10.00%;以其中1台仪器作为参比仪器,相关分析结果显示2台三分群血细胞分析仪WBC、RBC、Hct、Hb、Plt的相关系数(r)都在0.9 908~0.9 987之间,差异无统计学意义(P>0.05)。结论定期采用新鲜全血标本在不同分析仪器之间进行可比性分析可以发现系统偏差,三分群与五分类血细胞分析仪WBC、RBC、Hct、Hb、P1t 5个参数的结果一致性较好,而白细胞分类结果则存在一定的偏差,要根据病情并结合仪器直方图变化,制作血片以手工分类。
Objective To investigate the accuracy and comparability of major projects of multiple blood cell analyzers in one department. Methods According to the scheme developed by the American Committee for Clinical Laboratory Standards for the comparison and deviation assessment of patient samples, the results of the three hematology analyzers were analyzed and the systematic errors were found in time. Results The CV values of WBC, RBC, Hct, Hb and Plt in the three hematology analyzers were very close and the CV values were all less than 5.00%. The relative deviation of the percentage of mononuclear cells were -24.10% and -20.90% , The median and the low values are also close to -10.00%; one instrument as a reference instrument, the correlation analysis showed that two sets of three groups of blood cell analyzer WBC, RBC, Hct, Hb, Plt correlation coefficient (r) Between 0.9908 and 0.9987, the difference was not statistically significant (P> 0.05). Conclusion The systematic analysis of fresh blood samples from different whole blood samples can be used to find out the systematic deviations. The results of three parameters of three groups and five groups of blood cell analyzers WBC, RBC, Hct, Hb and P1t are consistent. Leukocyte classification results there is a certain deviation, according to the disease and changes in the instrument histogram, the production of blood films by manual classification.