该研究根据Ty1-copia类反转录转座子RT的通用引物,从铁皮石斛浙江临安(C15)和云南广南(A39)种质中扩增得到43条Ty1-copia类反转录转座子RT序列,这些核苷酸序列具有较高的异质性,主要表现为,序列长度变化范围260~266 bp,终止密码子和移码突变,所有序列均富含碱基AT,一致性为47.1%~97.7%。经plantCARE软件分析发现受低温、热、光、各种植物生长调节物质等不同胁迫条件作用的调控元件、多个启动子的特征结构TATA box和CAAT box的保守序列及其他一些调控元件。翻译成氨基酸后,有10条序列出现1~4个不同程度的终止密码子突变,5条序列出现移码突变,保守序列“SLYGKQ”发生变异的有39条序列。其氨基酸序列经过系统聚类后可分为6类;且与其他植物(尤其是单子叶植物的小麦、荸荠)具有较高的同源性,表明它们间可能存在着Ty1-copia反转录转座子的横向传递。 In this study, 43 Ty1-copia reverse transcripts were amplified from the germplasms of Dendrobium Lin Zhejiang (C15) and Yunnan Guangnan (A39) based on the universal primers of the Ty1-copia retrotransposon RT Sub-RT sequences. These nucleotide sequences have high heterogeneity, mainly showing sequence length ranging from 260 to 266 bp, stop codon and frameshift mutation. All sequences are rich in base AT, and the identity is 47.1% ~ 97.7%. The plant CARE software analysis found that by low temperature, heat, light, a variety of plant growth regulating substances under different stress conditions regulatory elements, a number of promoter features TATA box and CAAT box conserved sequences and other regulatory elements. After translated into amino acids, there were 10 stop codon mutations in 1 ~ 4 different sequences and 5 frameshift mutations. There were 39 sequences with conservative sequence “SLYGKQ”. Its amino acid sequence can be divided into 6 categories after being clustered systematically and has high homology with other plants (especially monocotyledonous wheat and water chestnut), indicating that there may exist Ty1-copia reverse transcription Block the horizontal transfer.