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为提高玉米秸秆厌氧发酵的产气效果,本文研究活性炭在中温(38℃)和高温(50℃)条件下对玉米秸秆厌氧发酵产甲烷的效果及微生物学机制.结果表明,添加活性炭能显著促进秸秆厌氧发酵产甲烷,中、高温试验组(添加活性炭)的累积产甲烷量分别比对照提高了63%和96%;DGGE的结果显示,高温试验组(添加活性炭)和对照组(未添加活性炭)的发酵液中的优势细菌菌群分别是Clostridiale bacterium和Bacillus,中温对照组发酵液和中温试验组发酵液未发现明显优势菌种.添加活性炭分别有利于氢营养型的甲烷鬃毛菌(Methanosaeta concilii strain)和乙酸营养型的醋酸甲烷八叠球菌(Methanosarcina acetivorans strain),在中温、高温试验组的发酵液中形成优势古菌菌群.中温试验组活性炭载体上的优势古菌菌群为甲烷鬃毛菌(Methanosaeta concilii strain),而高温试验组活性炭载体上的优势古菌菌群主要为甲烷八叠球菌嗜高温菌属(Methanosarcina thermophila strain).
In order to improve the effect of anaerobic fermentation of corn stover on gas production, this paper studies the effect and microbial mechanism of anaerobic fermentation of corn stover on methane production at activated carbon (38 ℃) and high temperature (50 ℃) .The results show that adding activated carbon The results showed that the cumulative methane yield of the experimental group fed with anaerobic fermentation of straw was 63% and 96% higher than that of the control, respectively. The results of DGGE showed that the high-temperature test group (activated carbon) and the control group No activated carbon) fermentation broth of the dominant bacteria were Clostridiale bacterium and Bacillus, medium temperature control group fermentation broth and medium temperature test group fermentation broth found no obvious dominant strains.Adding activated carbon, respectively, is conducive to hydrogen nutrient methane mane Methanosaeta concilii strain and Methanosarcina acetivorans strain were cultured in medium and high temperature test group, The group is Methanosaeta concilii strain, while the dominant archaeal flora on the activated carbon carrier in the high-temperature test group is mainly methane Methanosarcina thermophila strain.