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目的研究癫痫清颗粒中β-细辛醚在大鼠体内的药代动力学。方法用HPLC法测定大鼠体内不同时间的β-细辛醚的血药浓度,用DAS软件进行药代动力学分析。结果建立了用HPLC法测定β-细辛醚血药浓度的方法,大鼠口服癫痫清颗粒后,β-细辛醚在体内的药时过程为线性动力学过程,符合一级吸收二室模型,t1/2:8.238 h,Cmax:1.88 mg·L-1,Tmax:0.167h,AUC0-t:2.467mg·h-1·L-1,AUC0-∞:3.32 mg·h-1·L-1。结论实验方法简便、快速、灵敏,血浆中内源性物质及复方中各组分药物不干扰测定,可为癫痫清颗粒临床和量化研究提供可靠的依据。
Objective To study the pharmacokinetics of β-asarone in epilepsy granules in rats. Methods The plasma concentrations of β-asarone in rats at different times were determined by HPLC. The pharmacokinetics were analyzed by DAS software. Results The method of determining β-asarone serum concentration by HPLC was established. After oral administration of epilepsy granules, the pharmacokinetics of β-asarone in rats was linear kinetics, which accorded with the first-order absorption two-compartment model , t1 / 2: 8.238 h, Cmax: 1.88 mg · L-1, Tmax: 0.167 h, AUC0-t: 2.467 mg · h-1 · L-1 and AUC0-∞: 3.32 mg · h-1 · L- 1. Conclusion The experimental method is simple, rapid and sensitive. The determination of the endogenous substances in plasma and the drugs in the compound does not interfere with the determination, which can provide a reliable basis for the clinical and quantitative research of epilepsy granules.