【摘 要】
：
As specific pathogens of noctuid pests,including Spodoptera exigua,S.litura,Helicoverpa armigera,and Mythimna separata,ascoviruses are suitable for the development of bioinsecticides.In this study,the infectivity of Heliothis virescens ascovirus 3j (HvAV-
【机 构】
：
Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Ag
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As specific pathogens of noctuid pests,including Spodoptera exigua,S.litura,Helicoverpa armigera,and Mythimna separata,ascoviruses are suitable for the development of bioinsecticides.In this study,the infectivity of Heliothis virescens ascovirus 3j (HvAV-3j) on insect and mammalian cells was evaluated.HvAV-3j infection induced drastic morphological changes in Sf9,HzAM1,SeFB,and HaFB cells,including swelling and detachment.Notably,the latter phenomena did not occur in HvAV-3j-inoculated mammalian cells (HEK293,7402,HePG2,PK15,ST,and TM3).MTT assays indicated that HvAV-3j inhibited the growth of host insect cells from the 6th hpi,but no effects were detected in the HvAV-3j-inoculated mammalian cells.Furthermore,viral DNA replication,gene transcription,and protein expression were investigated,and the results consistently suggested that HvAV-3j viruses were not able to replicate their genomic DNA,transcribe,or express their proteins in the non-target vertebrate cells.The HvAV-3j genes were only transcribed and expressed in the four insect cell lines.These results indicated that HvAV-3j was infectious to cells derived from S.frugiperda,S.exigua,H.armigera,and H.zea but not to cells derived from human,pig,and mouse,suggesting that ascoviruses are safe to non-target vertebrate cells.
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