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目的探讨姜黄素(Curcumin)对睡眠剥夺(sleep deprived,SD)小鼠学习记忆力的影响。方法将50只C57雄性小鼠随机分为正常睡眠组、睡眠剥夺模型组和姜黄素低、中、高剂量组。采用改良多平台水环境法进行睡眠剥夺,姜黄素低、中、高剂量组分别于24、48及72 h经小鼠腹腔注射给药,剂量分别为5、10、20 mg/kg,正常睡眠组及睡眠剥夺模型组小鼠在相同时间给予腹腔注射等体积的生理盐水。每天给药1 h后经Morris水迷宫试验测试各组小鼠的学习记忆力;睡眠剥夺72 h后,检测小鼠海马组织中的超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)、丙二醛(malonaldehyde,MDA)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)的含量,Western blot法检测TNF-α的表达水平。结果与睡眠剥夺模型组比较,姜黄素各剂量组小鼠在Morris水迷宫定位航行试验中逃避潜伏期和游泳路程均明显缩短(P<0.05),空间探索试验中穿越平台次数明显增多(P<0.05);小鼠海马组织中SOD、GSH-Px含量明显增加(P<0.05),MDA、TNF-α含量明显降低(P<0.05);Western blot法结果表明,TNF-α的表达量明显下降(P<0.05)。结论姜黄素可改善睡眠剥夺小鼠学习记忆力,其机制可能是减少自由基堆积,增强抗氧化能力,减少炎症因子TNF-α的产生。
Objective To investigate the effect of curcumin on learning and memory in sleep deprived (SD) mice. Methods Fifty male C57 mice were randomly divided into normal sleep group, sleep deprivation model group and curcumin low, medium and high dose groups. The sleep deprivation was performed by a modified multi-platform water environment method. The curcumin low, medium and high dose groups were intraperitoneally injected with mice at 24, 48 and 72 h respectively at doses of 5, 10 and 20 mg / kg, Group and sleep deprivation model mice were injected intraperitoneally with equal volume of normal saline at the same time. The mice in each group were tested for learning and memory by Morris water maze test after 1 h of daily administration. After sleep deprivation for 72 h, the superoxide dismutase (SOD) and glutathione The levels of serum glutathione peroxidase (GSH-Px), malonaldehyde (MDA) and tumor necrosis factor-α (TNF-α) were determined by Western blot. . Results Compared with the sleep deprivation model group, the mice in curcumin groups had significantly shorter escape latency and swimming distance in the Morris water maze positioning test (P <0.05), and significantly increased the number of crossing platforms in the space exploration test (P <0.05 ). The content of SOD and GSH-Px in the hippocampus of mice increased significantly (P <0.05) and the content of MDA and TNF-α decreased significantly (P <0.05). The result of Western blot showed that the expression of TNF- P <0.05). Conclusion Curcumin can improve learning and memory of mice after sleep deprivation, the mechanism may be to reduce the accumulation of free radicals, enhance antioxidant capacity, reduce the production of inflammatory cytokine TNF-α.