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目的:探讨GP73(Golgi protein-73)在肝细胞癌诊断中的价值。方法:收集外周血血清504例,其中肝细胞癌144例、肝硬化50例、乙型病毒性肝炎100例、乙型肝炎病毒携带者84例、其他恶性肿瘤50例、肝良性肿瘤26例和健康志愿者50例;应用双抗体夹心酶联免疫定量测定方法和电化学发光法检测血清中GP73和甲胎蛋白(α-fetoprotein,AFP)的表达水平。结果:肝细胞癌组血清GP73的表达水平显著高于其他各组(P<0.05);受试者工作特征(receiver operating characteristic,ROC)曲线设定GP73临界值为64ng/mL时,GP73诊断肝细胞癌的灵敏度和特异度分别为83.3%和88.3%,显著高于AFP(72.2%和76.7%),差异有统计学意义(P<0.05);血清GP73联合AFP检测诊断肝细胞癌的灵敏度可达94.4%。结论:血清GP73的表达水平用于诊断肝细胞癌的灵敏度和特异度优于AFP,血清GP73联合AFP检测可提高肝细胞癌的诊断率。
Objective: To investigate the value of GP73 (Golgi protein-73) in the diagnosis of hepatocellular carcinoma. Methods: 504 cases of peripheral blood serum were collected, including 144 cases of hepatocellular carcinoma, 50 cases of cirrhosis, 100 cases of hepatitis B virus, 84 cases of hepatitis B virus carriers, 50 cases of other malignant tumors, 26 cases of benign liver tumors and Fifty healthy volunteers were enrolled in this study. Serum levels of GP73 and α-fetoprotein (AFP) were measured by double antibody sandwich enzyme-linked immunosorbent assay and electrochemiluminescence. Results: The expression level of GP73 in hepatocellular carcinoma was significantly higher than that in other groups (P <0.05). When receiver operating characteristic (ROC) curve was used to set the cutoff value of GP73 to 64 ng / mL, The sensitivity and specificity of cell carcinoma were 83.3% and 88.3%, respectively, which were significantly higher than those of AFP (72.2% and 76.7%) (P <0.05). The sensitivity of serum GP73 and AFP in diagnosis of hepatocellular carcinoma Up to 94.4%. Conclusion: The sensitivity and specificity of serum GP73 expression level for diagnosis of hepatocellular carcinoma are better than AFP. Serum GP73 combined with AFP detection can improve the diagnostic rate of hepatocellular carcinoma.