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基于腺酶催化尿素分解产生氨,以氨气敏电极为基础电极,用含脲酶丰富的谷氨酸棒状杆菌研制成测定尿素的微生物传感器。在30℃、pH8.0、0.1mol/L磷酸盐缓冲液中,该传感器的线性范围为1.1×10 ̄(-4)~1.4×10 ̄(-2)mol/L,斜率为51.2mV/decade,检测下限为1.0×10 ̄(-5)mol/L,寿命可达45d.考察了传感器响应初速和底物浓度之间的关系,测定了微生物膜中脲酶的表观米氏常数K_m及最大响应初速v_m.
Based on the ammonia-catalyzed decomposition of urea to produce ammonia, ammonia-based electrode as the base electrode, urease-rich Corynebacterium glutamicum was developed into the determination of urea microbial sensor. The linear range of this sensor was 1.1 × 10 -4 to 1.4 × 10 -2 mol / L in pH 8.0 and 0.1 mol / L phosphate buffer solution at 30 ℃. The slope is 51.2mV / decade, the detection limit is 1.0 × 10 ~ (-5) mol / L and the life span is up to 45 days. The relationship between the initial velocity of the sensor and substrate concentration was investigated. The apparent Michaelis constant K_m and the maximum response velocity v_m of urease in the microbial membrane were measured.