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目的检测增殖性玻璃体视网膜病变（ｐｒｏｌｉｆｅｒａｔｉｖｅｖｉｔｒｅｏｒｅｔｉｎｏｐａｔｈｙ，ＰＶＲ）发生过程中玻璃体内白细胞介素６（ｉｎｔｅｒｌｅｕｋｉｎ－６，ＩＬ－６）的含量变化，探讨其在ＰＶＲ早期免疫反应中的作用。方法用同种巨噬细胞诱发兔眼ＰＶＲ，在不同时间点抽取玻璃体液，采用双抗体夹心法ＥＬＩＳＡ试剂盒检测ＩＬ－６含量。结果ＩＬ－６含量１４ｄ达高峰９９８ｐｇ／ｍｌ，２８ｄ降至其基线水平；拟合二次曲线提示玻璃体中ＩＬ－６含量在１０～２１ｄ呈一高水平平台２５１ｐｇ／ｍｌ。结论ＩＬ－６在此ＰＶＲ模型中玻璃体含量在第２周明显升高，并持续至第３周，与ＰＶＲ形成中的第二阶段细胞增殖期相一致，提示ＩＬ－６对此ＰＶＲ模型细胞增殖、特别是对免疫活性细胞具有调控作用。
Objective To investigate the changes of intravitreal interleukin-6 (IL-6) in proliferative vitreoretinopathy (PVR) and its role in early PVR immune response. Methods The PVR of rabbit eyes was induced by allogeneic macrophages. Vitreous humor was drawn at different time points. IL-6 levels were detected by double antibody sandwich ELISA kit. Results The level of IL-6 reached a peak of 998 pg / ml on the 14th day and dropped to its baseline level on the 28th day. The fitting quadratic curve suggested IL-6 content in the vitreous showed a high level platform of 251 pg / ml from 10 to 21 days. CONCLUSION: The content of IL-6 in this PVR model increased significantly in the second week and lasted for the third week, which was consistent with the second phase of PVR formation. It suggested that IL-6 could promote the proliferation of PVR model cells , Especially on immune-competent cells.