论文部分内容阅读
将通过噬菌体显示技术获得的一株人源性抗HBsFab抗体基因重链和k轻链的可变区用编码柔性肽段的Linker使其连接成单链 ,克隆入中间载体 ,并进行核酸序列测定 ,为其后的表达及双功能抗体的构建打下基础。
A human anti-HBs Fab antibody gene heavy chain and a k light chain variable region obtained by phage display technology were ligated into a single strand with a linker encoding a flexible peptide, cloned into an intermediate vector, and the nucleic acid sequence was determined , Laying the foundation for the subsequent expression and the construction of bifunctional antibodies.