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目的研究染料木素在乳腺癌细胞中的代谢行为。方法将10μmol.L-1染料木素与乳腺癌MCF-7细胞在37℃下孵化一定时间后终止反应。采用UPLC-MS-MS法测定孵化液中染料木素及II相代谢产物的浓度。结果染料木素在乳腺癌MCF-7细胞中可迅速代谢为7或4′-葡萄糖醛酸结合物以及7或4′-硫酸结合物。孵化24、48和72 h后,每100μg孵化样品蛋白中,7-葡萄糖醛酸结合物的生成率分别为2.9、5.2和8.9μg.h-1,4′-葡萄糖醛酸结合物的生成率分别为1.1、1.9和3.0μg.h-1,7-硫酸结合物的生成率分别为20.3、32.9和47.3μg.h-1,4′-硫酸结合物的生成率分别为1.7、3.1和4.5μg.h-1。结论染料木素在乳腺癌MCF-7细胞中的主要代谢产物是7-硫酸结合物。
Objective To study the metabolic behavior of genistein in breast cancer cells. Methods 10μmol.L-1 genistein and MCF-7 breast cancer cells were incubated at 37 ℃ for a certain period of time to stop the reaction. UPLC-MS-MS was used to determine genistein and phase II metabolite concentrations in the incubation solution. Results Genistein was quickly metabolized to 7 or 4’-glucuronic acid conjugates and 7 or 4’-sulfate conjugates in breast cancer MCF-7 cells. The yields of 7-glucuronide conjugates were 2.9, 5.2 and 8.9 μg per 100 μg hatched sample protein at 24, 48 and 72 h after hatching, respectively. The rate of formation of the h-1,4’-glucuronide conjugate The rates of formation of the 1.1, 1.9 and 3.0 μg.h-1,7-sulfate conjugates were 20.3, 32.9 and 47.3 μg, respectively. The yields of the h-1,4’-sulfate conjugates were 1.7, 3.1 and 4.5, respectively μg.h-1. Conclusion The main metabolite of genistein in breast cancer MCF-7 cells is the 7-sulfate conjugate.