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本文报告1例来源于云南宣威县患者的肺癌标本,经体外培养建系成功,命名为SLC-89。该细胞系细胞经HE,瑞氏染色形态符合癌细胞特征。在体外培养已两年多,传代196代,细胞冻存后复苏生长良好。第86代细胞倍增时间为26.4小时,染色体数为非整倍体,众数为超二倍体,长期培养后染色体数明显增加。细胞接种裸鼠有移植瘤生长,组织象与原发肺癌组织象相似。电镜观察细胞表面有微绒毛,浆中可见分泌颗粒,有较多板层小体,表明来源于肺泡上皮。
This paper reports a case of lung cancer specimens from patients in Xuanwei County, Yunnan Province, successfully established in vitro culture system, named SLC-89. The cell line by HE, Wright staining consistent with cancer cell characteristics. In vitro cultured more than two years, passage 196 generation, cell recovery after cryopreservation well. The 86th generation cell doubling time was 26.4 hours, the chromosome number was aneuploid, the mode was hyperdiploid, and the chromosome number increased obviously after long-term culture. Nude mice inoculated with nude mice have xenograft tumors that resemble those of primary lung cancer. Electron microscopy cell surface microvilli, the secretion of particles visible in the pulp, there are more lamellar bodies, indicating that derived from the alveolar epithelium.