论文部分内容阅读
目的:研究不同龋敏感儿童口腔变形链球菌的培养及鉴定。方法:随机选取92例3～5岁的高龋、中龋及无龋儿童,用无菌刮匙刮取龋坏附近的菌斑,无龋者刮取咬合面窝沟内以及邻面的菌斑,置于盛有0.5 ml硫乙醇酸盐转送液的EP管中,用液体石蜡20μl覆盖,封口,迅速转运至实验室。所有临床分离株经微量生化板进行鉴定,并用革兰氏染色法镜检,观察细菌形态,菌细胞形态呈短链状、成对状或分散排列。结果:92例合格对象中,最终分离得到变形链球菌的有59例(64.1%),其中高龋者26例(81.0%),中龋者21例(72.0%),无龋者12例(39.0%)。33例未培养出变形链球菌的个体中,有19例是无龋个体,8例是中龋个体,6例是高龋个体。结论:菌斑中变形链球菌的数量与龋病的流行病学关系并不完全存在着因果关系。
Objective: To study the culture and identification of Streptococcus mutans in different caries-sensitive children. Methods: Ninety-two children with caries, middle caries and caries-free from 3 to 5 years old were randomly selected. The plaque near the caries was scraped with a sterile curette. Plaques were placed in an EP tube filled with 0.5 ml thioglycolate transfer fluid, covered with liquid paraffin 20 μl, sealed and rapidly transferred to the laboratory. All clinical isolates were identified by micro-biochemical plate, and Gram stain microscopy to observe the bacterial morphology, bacterial cell morphology was short-chain, paired or scattered arrangement. Results: Of the 92 eligible subjects, 59 (64.1%) were positive for S. mutans, of whom 26 (81.0%) had caries, 21 (72.0%) had caries, 12 had caries 39.0%). Of the 33 individuals who did not develop S. mutans, 19 were cario-free individuals, 8 were cariogenic individuals and 6 were carious individuals. Conclusion: There is not a causal relationship between the quantity of Streptococcus mutans in plaque and the epidemiology of dental caries.