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研究了葡萄糖月桂酸酯的酶法合成与分离测定。以丙酮为溶剂,用Novozym435脂肪酶催化合成了葡萄糖月桂酸酯,经液质联用(LC-MS)分析,反应产物为葡萄糖单酯和二酯,没有三酯或其它多酯生成。实验并优化了薄层色谱(TLC)法分析葡萄糖月桂酸酯的展开剂条件,得到展开剂∶甲苯∶乙酸乙酯∶甲醇∶水=10∶40∶4.5∶1.0(V/V/V/V),在此展开条件下,葡萄糖、葡萄糖单酯、二酯1和二酯2的Rf值相差均较大,分别为:0.098、0.51、0.85和0.97。用此展开剂作为流动相,采用硅胶柱层析分离葡萄糖月桂酸单酯,经高效液相色谱(HPLC)蒸发光散射检测(ELSD)和液质联用(LC-MS)鉴定,证实分离得到高纯度的葡萄糖月桂酸单酯。
Enzymatic synthesis and separation of glucose laurate were studied. Glucose laurate was synthesized catalyzed by Novozym 435 lipase using acetone as solvent. The product was glucose monoesters and diesters by LC-MS. No triglycerides or other polyesters were formed. The developing conditions for the analysis of glucose laurate by thin layer chromatography (TLC) were experimentally and optimally obtained. The developing solvent was toluene: ethyl acetate: methanol: water = 10:40:4.5:1.0 (V / V / V / V ). Under this condition, the Rf values of glucose, glucose monoester, diester 1 and diester 2 are all larger, which are respectively 0.098, 0.51, 0.85 and 0.97. Using this developing solvent as the mobile phase, the monolaurin was separated by silica gel column chromatography and identified by high performance liquid chromatography (HPLC) evaporative light scattering detection and liquid chromatography-mass spectrometry (LC-MS) High purity glucose monolaurate.