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目的 探讨胶质瘤血瘤屏障体外模型的形态学特征。 方法 利用电镜技术观察血管内皮细胞ECV30 4 和C6 胶质瘤细胞共培养 (混合共培养、Transwell共培养、Transwell膜两面共培养 )后的内皮细胞的孔窗、内皮细胞之间的连接及内皮细胞与肿瘤细胞的相互关系、瘤细胞的“血管周足”等的形态学特征 ;并与 4例人脑胶质瘤组织标本的血瘤屏障进行比较。 结果 电镜下发现ECV30 4 细胞经与C6 细胞按 3种不同方式共培养汇合后均为无孔窗型内皮细胞 ,细胞间出现紧密连接 ;Transwell共培养的膜上C6 细胞未见伸出伪足突向膜的微孔 ;经膜两面共培养的瘤细胞则可通过Transwell的微孔突向内皮细胞侧 ,但瘤细胞未伸出伪足包绕内皮细胞或突入内皮细胞间隙 ,瘤细胞的“血管周足”不完整 ,后两者与脑胶质瘤组织标本的血瘤屏障的形态特征相类似。 结论 在体外经Transwell膜两面共培养的ECV30 4 和C6 胶质瘤细胞的系统可在一定程度模拟体内的血瘤屏障的形态学特征。
Objective To investigate the morphological features of gliomas in vitro in vitro. Methods Electron microscopy was used to observe the pore window of endothelial cells after ECV30 4 and C6 glioma cells co-culture (mixed co-culture, Transwell co-culture and Transwell membrane co-culture), the connection between endothelial cells and endothelial cells And the relationship between the tumor cells, tumor cells “vascular week foot” and other morphological characteristics; and 4 cases of human glioma tissue specimens of the blood-borne barrier were compared. Results ECV30 4 cells were co-cultured with C6 cells in three different ways under electron microscope. All of them were non-porous window type endothelial cells with close connection between cells. There was no pseudopodal protrusion in C6 cells transfected with Transwell To the membrane pores; tumor cells co-cultured on both sides of the membrane can Transwell micropores to the endothelial cell side, but the tumor cells did not reach the pseudopod surrounded the endothelial cells or into the gap between endothelial cells, tumor cells, “blood vessels Zhou full ”is not complete, the latter two with glioma tissue specimens similar to the morphological features of the blood-borne barrier. Conclusion The system of ECV30 4 and C6 glioma co-cultured with Transwell membrane in vitro can simulate the morphological features of the blood-borne barrier in vivo to a certain extent.