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目的：探讨二甲基亚硝胺（Ｎ－ｎｉｔｒｏｓｏｄｉｍｅｔｈｙｌａｍｉｎｅ，ＮＤＭＡ）和部分肝切除术（ｐａｒｔｉａｌｈｅｐａｔｅｃｔｏｍｙ，ＰＨ）对大鼠肝脏ＤＮＡ合成的影响。方法：用免疫组织化学双ＰＡＰ法检测大鼠肝组织５′－溴脱氧尿嘧啶（５′－ｂｒｏｍｏｄｅｏｘｙｕｒｉｄｉｎｅ，ＢｒｄＵ）标记细胞。结果：１次或４次ＮＤＭＡ作用后５小时至２周，明显可见ＢｒｄＵ标记的肝细胞和肝窦壁细胞，四次ＮＤＭＡ处理ＢｒｄＵ标记细胞数高于一次处理。ＰＨ加一次ＮＤＭＡ处理后５小时至１周内，两类细胞ＢｒｄＵ标记细胞数高于相应单纯ＮＤＭＡ处理。ＰＨ加四次ＮＤＭＡ处理后５小时，ＢｒｄＵ标记肝细胞数高于相应单纯ＮＤＭＡ处理。ＢｒｄＵ标记细胞主要分布在门管区周围。结论：ＮＤＭＡ可刺激肝细胞和肝窦壁细胞ＤＮＡ合成，ＰＨ和ＮＤＭＡ对肝细胞和肝窦壁细胞的ＤＮＡ合成有协同刺激作用。
Objective: To investigate the effects of N-nitrosodimethylamine (NDMA) and partial hepatectomy (PH) on hepatic DNA synthesis in rats. METHODS: The 5′-bromodeoxyuridine (BrdU)-labeled cells were examined by immunohistochemical double PAP. RESULTS: BrdU-labeled hepatocytes and hepatic sinusoidal parietal cells were clearly visible from 5 to 2 weeks after 1 or 4 NDMA exposures. The number of BrdU labeled cells treated with four NDMA treatments was higher than that of one treatment. Within 5 hours to 1 week after PH plus one NDMA treatment, the number of BrdU-labeled cells in both types of cells was higher than that of the corresponding NDMA alone. Five hours after PH plus four NDMA treatments, the number of BrdU-labeled hepatocytes was higher than that of the corresponding NDMA alone. BrdU-labeled cells are mainly distributed around the portal area. Conclusion: NDMA can stimulate DNA synthesis of hepatocytes and hepatic sinus wall cells. PH and NDMA have synergistic stimulatory effects on DNA synthesis of hepatocytes and hepatic sinusoidal cells.