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目的探讨可溶性肿瘤坏死因子相关凋亡诱导配体(soluble tumor necrosis factor-related apoptosis inducing ligand,sTRAIL)真核表达质粒对人胃癌裸鼠移植瘤的抑制作用。方法建立胃癌细胞株BGC-823裸鼠移植瘤模型,成瘤后于瘤内多点注射重组真核表达质粒pBud-EGFP-TRAIL,并设pBud-EGFP组和生理盐水组为对照,观察并记录肿瘤的生长情况。Western blot和RT-PCR检测sTRAIL mRNA和蛋白表达情况,HE染色观察肿瘤组织病理改变,TUNEL法及电镜技术观测肿瘤组织细胞凋亡情况。结果注射pBud-EGFP-TRAIL组的sTRAIL mRNA和蛋白为阳性,其瘤体大小明显小于pBud-EGFP组和生理盐水组,差异有统计学意义(P<0.05)。HE染色示pBud-EGFP-TRAIL组肿瘤细胞大量死亡,电镜下可见到典型凋亡细胞,TUNEL法显示其凋亡细胞明显增多。结论sTRAIL真核表达质粒pBud-EGFP-TRAIL瘤内注射对人胃癌裸鼠移植瘤有抑制作用。
Objective To investigate the inhibitory effect of soluble tumor necrosis factor-related apoptosis inducing ligand (sTRAIL) on human gastric cancer xenografts in nude mice. Methods The gastric cancer cell line BGC-823 xenografted in nude mice was established. After tumorigenesis, the recombinant eukaryotic expression plasmid pBud-EGFP-TRAIL was injected into the tumor and pBud-EGFP group and saline group were used as controls. Tumor growth. The expression of sTRAIL mRNA and protein were detected by Western blot and RT-PCR. The pathological changes of tumor tissue were observed by HE staining. TUNEL and electron microscopy were used to observe the apoptosis of tumor cells. Results The sTRAIL mRNA and protein in pBud-EGFP-TRAIL group were positive, and the tumor size was significantly smaller than that in pBud-EGFP group and saline group (P <0.05). HE staining showed that a large number of tumor cells died in pBud-EGFP-TRAIL group, and typical apoptotic cells were observed under electron microscope. TUNEL assay showed that apoptotic cells were significantly increased. Conclusion The sTRAIL eukaryotic expression plasmid pBud-EGFP-TRAIL intratumoral injection of human gastric cancer xenografts inhibited.