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用离体孵育的方法研究了睾酮 (T)对日本鳗鲡 (Anguillajaponica)完整的下丘脑 脑垂体复合物(hypothalamus pituitarycomplex ,HPC)、分离的下丘脑 (hypothalamus,H)加脑垂体 (pituitary ,P)以及单独的脑垂体 (pituitary ,P)促性腺激素 (GtH)合成与释放的影响。在不加T的孵育液中孵育 ,HPC组的孵育液及其脑垂体中的GtH含量最高 ,H +P组次之 ,而P组最低 ,表明下丘脑通过GnRH直接刺激脑垂体GtH的合成与释放。在加入T的孵育液中孵育 ,P组的孵育液及脑垂体中的GtH含量显著增加 ,并且和孵育液中的T呈剂量依存的正反馈作用。当T和H与P一起孵育时 ,低浓度的T (0 1和 1μmol/L)刺激HPC组和H +P组的GtH释放 ,呈现正反馈作用 ,而高浓度的T (10 μmol/L)则抑制HPC组和H +P组的GtH释放 ,表现负反馈作用。这些结果直接证明日本鳗鲡下丘脑对脑垂体GtH分泌的调控以及性类固醇激素 (如雄鳗的睾酮 )对脑垂体GtH分泌的反馈作用
The effects of testosterone (T) on intact hypothalamus pituitary complexes (HPC), isolated hypothalamus (H) and pituitary (P) in Japanese Anguilla japonica were studied by in vitro incubation. And the effects of pituitary (P) gonadotrophin (GtH) synthesis and release. In the incubation without T, HPT group had the highest GtH level in the incubation solution and pituitary gland, followed by H + P group and the lowest in P group, indicating that the hypothalamus directly stimulated the synthesis of pituitary GtH through GnRH and freed. After incubation with T, the content of GtH in P group and pituitary significantly increased, and dose-dependent positive feedback relationship with T in incubation solution. Low concentrations of T (0 1 and 1 μmol / L) stimulated the release of GtH from HPC and H + P groups when positive T and H were incubated with P, whereas high concentrations of T (10 μmol / L) The inhibition of GtH release in HPC group and H + P group showed a negative feedback effect. These results directly demonstrate the regulation of the secretion of GtH in the pituitary by Japanese eel (Anguilla japonicum) and the feedback effect of sex steroid hormones (such as testosterone in male eels) on the secretion of GtH in the pituitary gland