目的　建立测定家兔血浆中葛根黄豆苷元衍生物DZ18含量的高效液相色谱法。方法　采用HPLC法 ,使用ODS -C18色谱柱 ,流动相 :甲醇 -水 -磷酸 (5 0∶5 0∶0 .1) ,检测波长 2 6 0nm。结果　DZ18浓度在 0～ 10 0 μg·ml-1范围内 ,线性关系良好 ,r=0 .9998。回收率在 89%以上 ;日内、日间精密度均低于 5 %。结论　本方法为DZ18的药动学研究提供了可靠的检测手段 Objective To establish a HPLC method for the determination of daidzein derivative DZ18 in rabbit plasma. Methods HPLC method was used on ODS-C18 column. The mobile phase was methanol-water-phosphoric acid (50∶0.50∶1), detection wavelength was 260nm. Results The concentration of DZ18 was in the range of 0～10 0 μg·ml-1 with good linear relationship, r=0.9998. The recovery rate was above 89%; the intraday and daytime precisions were all below 5%. Conclusion This method provides a reliable detection method for the pharmacokinetic study of DZ18