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目的:揭示川乌煎煮时间长短、给药剂量与毒性的相关性。方法:将川乌分别煎煮15min,30min,1h,2h,3h,4h,5h,给药剂量依次为临床用量的5、20、40、64、96、110倍。选择复制麻痹症动物模型按组分别用药30天。用药的同时观测模型动物的生长发育、脏器系数、血液生化学、病理组织学变化以及停药30天后上述指标的变化。从结果中分析川乌煎煮时间的长短、给药剂量的多少与毒性大小的相关性。结果:川乌水煎液能够有效抑制痹证动物的足跖肿胀程度;明显影响了模型动物的生长发育;虽然对模型动物造血系统没有很明显的毒性,但是对模型动物的肝脏、心脏、肾脏能够产生一定的毒性。川乌在煎煮30min、给药剂量为110倍时其水煎液的毒性最大。结论:煎煮30min或给药剂量为临床常用量的110倍时,川乌的水煎液对麻痹动物模型产生的毒性最大,川乌的药效也为最佳状态。
OBJECTIVE: To reveal the relationship between the time of decoction of Radix et Rhizoma Rhei and dose and toxicity. Methods: Chuanwu were decocted for 15min, 30min, 1h, 2h, 3h, 4h, 5h, the dosage was 5,20,40,64,96,110 times of the clinical dosage. The animal models of replication paralysis were selected for 30 days. At the same time, we observed the growth and development, the organ coefficient, blood biochemistry, histopathological changes and the change of above indexes after 30 days of withdrawal. From the results of the analysis of Chuanwu decoction of the length of time, the number of doses and toxicity associated with the size. Results: Chuanwu decoction could effectively inhibit the paw edema of arthralgia animal and significantly affected the growth and development of model animals. Although there was no obvious toxicity on the model animals’ hematopoietic system, the liver, heart and kidney of model animals Can produce some toxicity. Chuanwu decoction 30min, the dose of 110 times the decoction of its toxicity. CONCLUSION: When decocted for 30 minutes or administered at a dose of 110 times of the commonly used clinical dose, the decoction of Radix Aconiti Preparata has the highest toxicity to paralyzed animal model and the efficacy of Radix Aconitum is optimal.