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为开发青藏高原沙地生态系统放线菌资源,首次从位于青藏高原东北部的青海湖沙岛土壤样品中选择性分离培养出一株具明显抑菌及抗肿瘤活性放线菌Sd-31.通过生理生化特性、化学分类学以及16S rRNA序列分析鉴定,菌株为砖红链霉菌,命名为Streptomyces lateritius Sd-31;双层琼脂法检测菌株抑菌活性,表明菌株对金黄色葡萄球菌(Staphyloccocus aureus)以及枯草芽孢杆菌(Bacillus subtilis)有显著抑制作用;通过Hoechst染色荧光显微镜观察及MTT法细胞毒性试验,确定菌株发酵液有明显的诱导肿瘤细胞凋亡活性且与处理浓度成正相关;纸片法研究菌株发酵液活性稳定性,结果表明,20~80℃处理后发酵液抑菌活性稳定,100℃条件处理后发酵液抑菌活性降低;在酸性及中性条件下发酵液活性稳定,碱性条件下随着pH值的增加发酵液抑菌活性降低.以上结果为抗肿瘤药物的研究提供了理论数据.
In order to develop actinomycetes resources in the sand ecosystem of the Qinghai-Tibet Plateau, a strain of actinomycetes Sd-31 with obvious antibacterial activity and antitumor activity was selectively isolated and cultured from soil samples from sand island of Qinghai Lake located in the northeastern Qinghai-Tibet Plateau. Staphylococcus aureus (Streptomyces rubrum) was identified by its physiological and biochemical characteristics, chemical taxonomy and 16S rRNA sequence analysis. The strain was named Streptomyces latercine Sd-31. The antibacterial activity of Staphyloccocus aureus ) And Bacillus subtilis were significantly inhibited. Hoechst staining and MTT cytotoxicity assays showed that the fermentation broth of the strain had significant apoptosis-inducing activity and was positively correlated with the treatment concentration. The results showed that the antibacterial activity of the fermentation broth was stable after treatment at 20-80 ℃, and the antibacterial activity of the fermentation broth decreased after treatment at 100 ℃. The activity of the fermentation broth was stable under acidic and neutral conditions, The antibacterial activity of the fermentation broth decreased with the increase of pH value.The above results provide theoretical data for the study of antitumor drugs.