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本文采用SPA-ELISA法评价了三种不同纯度牛源细粒棘球蚴囊液抗原在包虫病免疫诊断中的价值,用三种抗原检测了41例包虫病患者血清中特异IgG抗体的水平,它们的阳性率分别为:牛源细粒棘球蚴液粗抗原86.0％,半饱和硫酸铵法纯化抗原91.4％,氯化镁—磷钨酸法纯化抗原92.3％,两种纯化抗原与粗抗原的阳性检出率相比,经统计学处理具有显著差异,认为西北地区牦牛体内寄生的细粒棘球蚴囊液抗原不乏抗原活性;氯化镁—磷钨酸法和半饱和硫酸铵法是两种简便易行,纯化效果理想的抗原纯化方法,宜于在基层推广应用。
In this paper, the SPA-ELISA method to evaluate the three different purity of bovine cyst fluid antigen analysis of echinococcosis in the immunodiagnosis value of three antigens detected 41 cases of echinococcosis patients serum specific IgG antibody The positive rates were 86.0% for crude Echinococcus granulosus solution, 91.4% for half-saturated ammonium sulfate method and 92.3% for purified antigen by magnesium chloride-phosphotungstic acid method, respectively. The two purified antigen and crude antigen Compared with the positive detection rate compared to the statistical treatment of significant differences that the yak in Northwest parasites in the absence of antigen hydatid cyst fluid antigen; magnesium chloride - phosphotungstic acid method and half-saturated ammonium sulfate method are two Simple and easy to purify the effect of the ideal antigen purification method, suitable for promotion and application in the grassroots.